Critical comparison of ELISA kits for CGRP measurements in migraine.

We studied several commercial ELISA kits to measure CGRP levels in migraine. CGRP is a key biomarker linked to migraines, but its measurement reliability is debated. We tested serum samples from 19 migraine patients at a hospital. We also used synthetic CGRP samples at various concentrations. Four ELISA kits were evaluated: one for α-CGRP and three for β-CGRP. Each kit was assessed following the manufacturer's instructions. Our findings suggest that the performance of these kits varies significantly. This variability raises questions about the accuracy of CGRP measurements in clinical settings. Reliable CGRP measurement is crucial for understanding migraine mechanisms and developing effective treatments. Further research is needed to determine which kits provide the most consistent results. This study highlights the importance of validating measurement tools in migraine research and clinical practice for better patient outcomes. Clinical relevance: This research emphasizes the need for reliable CGRP measurement tools in migraine treatment.